EGFR polyclonal antibody
产品名称: EGFR polyclonal antibody
英文名称: EGFR polyclonal antibody
产品编号: PAB2080
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of EGFR.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to residues surrounding Y1092 of human EGFR.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein A purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Distinct domains in the adenovirus E3 RIDalpha protein are required for degradation of Fas and the epidermal growth factor receptor.
Zanardi TA, Yei S, Lichtenstein DL, Tollefson AE, Wold WS.J Virol. 2003 Nov;77(21):11685-96.
- 2.
- Tyrosine phosphorylation of the beta2 subunit of clathrin adaptor complex AP-2 reveals the role of a di-leucine motif in the epidermal growth factor receptor trafficking.
Huang F, Jiang X, Sorkin A.J Biol Chem. 2003 Oct 31;278(44):43411-7. Epub 2003 Aug 4.
- 3.
- Aldosterone stimulates epidermal growth factor receptor expression.
Krug AW, Grossmann C, Schuster C, Freudinger R, Mildenberger S, Govindan MV, Gekle M.J Biol Chem. 2003 Oct 31;278(44):43060-6. Epub 2003 Aug 25.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of MDA-MB-231 cell lysate (35 ug/lane) with EGFR polyclonal antibody (Cat # PAB2080).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EGFR polyclonal antibody (Cat # PAB2080) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Entrez GeneID:
- 1956
- Protein Accession#:
- NP_005219;P00533
- Gene Name:
- EGFR
- Gene Alias:
- ERBB,ERBB1,HER1,PIG61,mENA
- Gene Description:
- epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
- Other Designations:
- avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor
- Interactome
- Gene Pathway
- Adherens junction
- Bladder cancer
- Calcium signaling pathway
- Colorectal cancer
- Cytokine-cytokine receptor interaction
- Dorso-ventral axis formation
- Endocytosis
- Endometrial cancer
- Epithelial cell signaling in Helicobacter pylori infection
- ErbB signaling pathway
- Focal adhesion
- Gap junction
- Glioma
- GnRH signaling pathway
- MAPK signaling pathway
- Melanoma
- Non-small cell lung cancer
- Pancreatic cancer
- Pathways in cancer
- Prostate cancer
- Regulation of actin cytoskeleton
- Related Disease
- Adenocarcinoma
- Adenocarcinoma, Bronchiolo-Alveolar
- Adenocarcinoma, Mucinous
- Adenocarcinoma, Papillary
- Anus Neoplasms
- Asthma
- Asthma
- Astrocytoma
- Atherosclerosis
- Barrett Esophagus
- Bile Duct Neoplasms
- Biliary Tract Neoplasms
- Bipolar Disorder
- Brain Neoplasms
- Breast cancer
- Breast Neoplasms
- Bronchial Hyperreactivity
- Carcinoma
- Carcinoma, Adenosquamous