Synonyms:	EFM-192C（人乳腺癌细胞）EFM-192C
Background:	established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma; sister cell lines EFM-192A and -192B show differences in molecular aspects; erbB-2 gene is amplified and overexpressed
Species:	human (Homo sapiens)
Tissue:	breast
Disease:	breast carcinoma
Gender:	N/A
Morphology:	epitheloid adherent cells growing as monolayers
Growth Mode:	N/A
Doubling Time:	ca. 4 days
DNA Profile:	N/A  Cell Line Authentication Service
Culture Medium:	RPMI-1640+20%FBS We strongly suggest to purchase the complete medium from
Cryopreservation medium:	90%FBS+10%DMSO
Mycoplasma:	negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:	cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin -
Fingerprint:	same DNA profile as EFM-192A/EFM-192B using multiplex PCR of minisatellite markers
Species:	confirmed as human with IEF of AST, MDH, NP
Cytogenetics:	highly rearranged human hypohexaploid karyotype with 10% polyploidy - 122-138<6n>XXXXX/XXXXXX - related to EFM-192A and EFM-192 B
Virus	ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -