DIG高效DNA标记及检测StarterKit 1 说明书

DIG-High Prime DNA Labeling and Detection Starter Kit I

Application

DIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Benefits

The High Prime method of labeling offers many benefits. Plasmid, cosmid, and lambda phage DNA have all been used as templates. We have achieved equal efficiency when labeling 10 ng to 3 µg of template DNA, which may be linear or supercoiled.

Product Description

Sensitivity and specificity: The standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, and 2.3 µg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

Contents

1.       DIG-High Prime, 5x conc., 50 µl

2.       DIG-labeled Control DNA, 20 µl, (5 µg/ml), pBR328 (linearized with Bam HI)

3.       DNA Dilution Buffer, 3 x 1 ml

4.       Anti-digoxigenin-AP Conjugate, 100 µl

5.       NBT/BCIP Stock Solution, conc., 6 x 1 ml

6.       Blocking Solution, 10x conc., 4 x 100 ml

DIG Easy Hyb Granules, 4 x 100 ml

DIG高效DNA标记及检测Starter Kit II说明书

DIG-High Prime DNA Labeling and Detection Starter Kit II

Application

DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Benefits

The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.

Product Description

Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

Contents

1.       DIG-High Prime, 5x conc., 50 µl

2.       DIG-labeled Control DNA, 20 µl, (5 µg/ml) pBR328 (linearized with Bam HI)

3.       DNA Dilution Buffer, 3 x 1 ml

4.       Anti-digoxigenin-AP Conjugate, 50 µl

5.       CSPD, ready-to-use, 50 ml

6.       Blocking Solution, 10x conc., 4 x 100 ml

7.       DIG Easy Hyb Granules, 4 x 100 ml