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Granulocyte-Colony Stimulating Factor Antibody
 
 
 
 
 
 
 
 
 
Order or Inquire for GCSF Antibody product
 
 
 
 
 
 
 
Detection limit is 1 ng/lane in WB
 
 
 
 
 
 
G-CSF / GCSF抗体产品信息
 
 
 
 
免疫原 : 
 
 
Recombinant human G-CSF protein ( Catalog#10007-HNAE )
 
 
 
 
抗体类型: 
 
Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
 
 
 
 
克隆号 :
 
 
 
9A6D9C4
 
 
 
 
抗体宿主 :
 
 
Mouse IgG2a
 
 
 
 
缓冲液 :
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
制备方法 : 
 
 
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human G-CSF ( rh G-CSF ; Catalog#10007-HNAE ; NP_757373.1 ; Ala 30 - Pro 204 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 
 
 
 
 
G-CSF / GCSF抗体使用指南
 
 
 
 
 
特异性 : 
 
 
 
Human G-CSF / GCSF
 
 
 
 
 
 
No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
 
 
 
Western blot : 
 
This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human G-CSF in WB. Using a DAB detection system, the detection limit for human G-CSF is approximately 1 ng/lane under non-reducing conditions and reducing conditions
 
 
 
储存方法 :
 
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
 
 
 
 
G-CSF / GCSF Antibody Related Products & Topics
 
 
相关研究领域:
 
Cancer>>Growth Factor & Receptor>>CSF & Receptor>>G-CSF/CSF3
 
Immunology>>Cytokine & Receptor>>CSF & Receptor>>G-CSF/CSF3
 
Immunology>>Cytokine & Receptor>>Interleukin & Receptor>>IL-6 Family Signaling Related Molecules>>G-CSF/CSF3
 
蛋白:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
G-CSF/CSF3
 
Human
 
G-CSF/CSF3/Fc (isoform b) Protein, Recombinant
 
10007-H01H
 
 
 
G-CSF/CSF3
 
Human
 
G-CSF/CSF3 (isoform b) Protein, Recombinant
 
10007-HNAE
 
 
 
 
抗体:
 
 
 
 
Molecule
 
Application
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Human 
 G-CSF/CSF3
 
WB
 
Mouse Monoclonal Antibody
 
10007-MM05
 
 
 
Human 
 G-CSF/CSF3
 
ELISA
 
Mouse Monoclonal Antibody
 
10007-MM07
 
 
 
Human 
 G-CSF/CSF3
 
WB, ELISA
 
Rabbit Polyclonal Antibody
 
10007-RP01
 
 
 
Human 
 G-CSF/CSF3
 
WB, ELISA
 
Rabbit Polyclonal Antibody (Antigen Affinity Purified)
 
10007-RP02
 
 
 
 
 
 G-CSF / GCSF抗体背景综述
 
 
Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins that regulate haematopoietic cell proliferation and differentiation. G-CSF was isolated initially as a factor supporting the growth of colonies of granulocytes in soft agar cultures. Cells of the monocyte/macrophage lineage are among the most prominent sources of G-CSF, but this factor can also be produced by normal cells of mesodermal origin, including vascular endothelial cells, fibroblasts, and mesothelial cells. Production of G-CSF can be induced in vitro in these cells by a wide variety of stimulatory agents, including LPS, TNF, IL-1, IL-3, I L-4, and IFN-γ. G-CSF is likely to play a role in the basal regulation of neutrophil production, and also functions as a primary regulatory factor controlling the neutrophil response to inflammatory stimuli. Furthermore, G-CSF exhibits other biological activities besides the proliferative effects, since G-CSF appears to modulate the distribution of neutrophils and progenitor cells within the body.
 
 
参考文献
 
 
 
Khanna-Gupta A, et al., 2006, J Leukoc Biol. 79(5):1011-21.
 
Nagata, et al., 1986, Nature. 319: 415-418.
 
George D. Demetri, et al., 1991, Blood, Vol78, No 11: 2791-2808.
 
 
 
 
 
 
 
 
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