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上海信裕生物科技有限公司
JUNB抗体

JUNB抗体

商家询价

产品名称: JUNB抗体

英文名称: JUNBAntibody

产品编号: XY 10486-1

产品价格: null

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
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JUNB抗体

Applications

In-house applications ELISA, WB, IHC, IF, IP     Application not tested? Please see our Guarantee.
Species specificity Human,Mouse,Rat; other species not tested.
Images
Prev12NextView more validation data
  • 10486-1-AP;MCF7 cellsMCF7 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP(JUNB antibody) at dilution of 1:500
  • 10486-1-AP;human gliomasImmunohistochemical of paraffin-embedded human gliomas using 10486-1-AP(JUNB antibody) at dilution of 1:50 (under 25x lens)
  • 10486-1-AP;HeLa cellsIP Result of anti-JUNB (IP:10486-1-AP, 4ug; Detection:10486-1-AP 1:500) with HeLa cells lysate 1200ug.
  • 10486-1-AP;MCF-7 cellsImmunofluorescent analysis of MCF-7 cells using 10486-1-AP( JUNB Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in MCF7 cells, C6 cells, HeLa cells, HT-1080 cells
Positive IP detected in HeLa cells
Positive IHC detected in Human gliomas tissue, human ovary tissue, human testis tissue
Positive IF detected in MCF-7 cells, HeLa cells
Recommended dilution WB : 1:200-1:2000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100
JUNB抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen JUNB fusion protein ag0752 Full name Jun B proto-oncogene
Calculated molecular
weight
36 kDa Observed molecular 
weight
42 kDa, 43 kDa
GenBank accession 
number
BC004250 Gene ID (NCBI) 3726
Gene symbol JUNB Synonyms AP 1, jun B proto oncogene, JUNB, Transcription factor jun B
BackgroundJUNB抗体

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological contextJUNB抗体

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