FITC标记的细胞核受体ERBA相关基因2抗体
产品名称： FITC标记的细胞核受体ERBA相关基因2抗体
英文名称： Anti-NR2F6/FITC
产品编号： HZ-
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： Flow-Cyt=1ug/Test ICC=1:50-200 IF=1:50-200
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Rabbit Anti-NR2F6/FITC Conjugated antibody
FITC标记的细胞核受体ERBA相关基因2抗体
| 英文名称 | Anti-NR2F6/FITC |
| 中文名称 | FITC标记的细胞核受体ERBA相关基因2抗体 |
| 别 名 | EAR 2; EAR-2; EAR2; ERBA RELATED 2; ERBA related gene 2; ERBAL2; Nr2f6; NR2F6_HUMAN; Nuclear receptor subfamily 2 group F member 6; Orphan nuclear receptor EAR2 (V erbA related protein EAR 2); v erb a avian erythroblastic leukemia viral oncogene homolog like; V erbA related protein EAR 2; V-erbA-related protein 2. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 染色质和核信号 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Cow, Horse, Sheep, |
| 产品应用 | Flow-Cyt=1ug/Test ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 43kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NR2F6 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | Function: Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC). Subcellular Location: Nucleus. Tissue Specificity: Expressed in heart, placenta, liver, skeletal muscle, kidney and pancreas. Similarity: Belongs to the nuclear hormone receptor family. NR2 subfamily. Contains 1 nuclear receptor DNA-binding domain. Database links: Entrez Gene: 2063 Human Entrez Gene: 13864 Mouse Entrez Gene: 245980 Rat Omim: 132880 Human SwissProt: P10588 Human SwissProt: P43136 Mouse SwissProt: O09017 Rat Unigene: 466148 Human Unigene: 28989 Mouse Unigene: 440170 Mouse Unigene: 25840 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Blank control (Black line): A431 (Black). Primary Antibody (green line): Rabbit Anti-NR2F6 antibody (bs-19340R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed |
转录因子主要参与转录抑制。与启动子/增强子反应元件结合，所述启动子/增强子反应元件包含不完全的5’-AGTCA-3’直接或倒置重复序列，具有其他核受体也识别的各种间隔。参与反应的调节。抑制黄体生成素-绒毛膜促性腺受体/LHCGR基因、肾素/REN基因和催产素-神经营养素/OXT基因的转录活性。以细胞类型特异性的方式抑制甲状腺受体基因的三碘甲状腺原氨酸依赖性和非依赖性转录活性。对甲状腺受体β/THRB的共抑制功能至少部分涉及NR2F6抑制THRB与三碘甲状腺原氨酸反应元件(TRE)的结合。抑制NFATC转录因子的DNA结合，并随后其转录活性。作为IL-17在Th-17分化的CD4(+)T细胞中表达的转录抑制因子，可能参与外周免疫耐受和自身免疫的诱导和/或维持。涉及到前脑生物钟的发展；在蓝斑（LC）的发展中是需要早期的。