Disease:

 Primary Pancreatic adenocarcinoma 

Origin:

 BxPC-3 cells produce mucin and the tumour produced in a nude mouse is moderately well to poorly differentiated like the primary adenocarcinoma 

Species:

 Homo sapiens 

Tissue:

 Pancreas 

Morphology:

 Epithelial 

Properties:

 Adherent 

Cytogenic data:

 Tumorigenic; 2n = 59, near triploid 

Patient:

 Female, 61 yrs of age 

Medium:

 RPMI 1640 + 10% FBS 

Subculture:

 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

 Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:
 I 
Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Protein Expression:
 mucin 
DNA Profile (STR):
 Amelogenin: X; CSF1PO: 13; D13S317: 11; D16S539: 9,11; D5S818: 11; D7S820: 10,13; THO1: 9; TPOX: 8; vWA: 14,18 
Mutations in BxPc-3 Cells 

Related cells by Organ:

AR-42J cells 

BxPc-3 cells 

Capan-1 cells 

MIA PaCa-2 cells 

E3LZ10.7 cells 

A10.7 cells 

INS-1 cells 

MIN-6 cells 


References for BxPc-3 cells:

1. Bernhaus, A., Ozsvar-Kozma, M., Saiko, P., Jaschke, M., Lackner, A., Grusch, M., Horvath, Z., Madlener, S., Krupitza, G., Handler, N., Erker, T., Jaeger, W., Fritzer-Szekeres, M., and Szekeres, T. Antitumor effects of KITC, a new resveratrol derivative, in AsPC-1 and BxPC-3 human pancreatic carcinoma cells. Invest New Drugs, 27: 393-401, 2009. 

2. Lu, Y. Y., Jing, D. D., Xu, M., Wu, K., and Wang, X. P. Anti-tumor activity of erlotinib in the BxPC-3 pancreatic cancer cell line. World J Gastroenterol, 14: 5403-5411, 2008. 

3. Nyaga, S. G., Lohani, A., and Evans, M. K. Deficient repair of 8-hydroxyguanine in the BxPC-3 pancreatic cancer cell line. Biochem Biophys Res Commun, 376: 336-340, 2008. 

4. Yeo, T. K., Kintner, J., Armand, R., Perez, R., and Lewis, L. D. Sublethal concentrations of gemcitabine (2',2'-difluorodeoxycytidine) alter mitochondrial ultrastructure and function without reducing mitochondrial DNA content in BxPC-3 human pancreatic carcinoma cells. Hum Exp Toxicol, 26: 911-921, 2007. 

5. Huang, Z., Guo, K. J., Guo, R. X., and He, S. G. Effects of 5-fluouracil combined with sulfasalazine on human pancreatic carcinoma cell line BxPC-3 proliferation and apoptosis in vitro. Hepatobiliary Pancreat Dis Int, 6: 312-320, 2007. 

6. Ahsan, H., Reagan-Shaw, S., Breur, J., and Ahmad, N. Sanguinarine induces apoptosis of human pancreatic carcinoma AsPC-1 and BxPC-3 cells via modulations in Bcl-2 family proteins. Cancer Lett, 249: 198-208, 2007.